Journal: Bioactive Materials

Article Title: A catalytically active and recyclable bioelastomer inspired by metalloenzymes

doi: 10.1016/j.bioactmat.2026.02.053

Figure Lengend Snippet: Cytocompatibility of Cu-PIAS. ( A ) Viability, as measured by ATP content, of human umbilical vein endothelial cells cultured with media containing extracts of 15-Cu-PIAS, Latex (Positive), or PCL (Negative) over three days. ( B ) Representative images of cell morphology after 24 h exposure to material extract-containing media, visualized by Calcein AM (Green) staining. Additional staining with ethidium homodimer-1 (red) indicates the presence of non-viable cells.

Article Snippet: Briefly, endothelial growth medium supplemented with 5% fetal bovine serum, growth factors, ascorbic acid, and hydrocortisone (Promocell C-22121) was incubated at 37 °C for 72 h under agitation alone, or in the presence of 15-Cu-PIAS (6 cm 2 /mL), PCL (6 cm 2 /mL), or latex (3 cm 2 /mL) sheets cut into several 6 mm × 6 mm squares.

Techniques: Cell Culture, Staining

Journal: Translational Oncology

Article Title: Integrated spatial and single‑cell transcriptomics maps disulfidptosis in renal cell carcinoma and reveals PDLIM1 as a prognostic biomarker and potential therapeutic target

doi: 10.1016/j.tranon.2026.102765

Figure Lengend Snippet: PDLIM1 knockdown reduces proliferation, clonogenicity, and migration in renal cancer cells in vitro. (A) qPCR validation of siRNA/shRNA‑mediated PDLIM1 knockdown in renal cancer cell lines (786‑O and OSRC-2). (B) CCK‑8 proliferation/viability curves comparing control and PDLIM1‑depleted cells over time. (C-D) Representative images (C) and quantification (D) of wound‑healing assays. (E) Transwell migration assays showing decreased motility/invasiveness after PDLIM1 depletion. (F) Quantification of transwell migration assays. Data are presented as mean ± SEM; statistical significance was determined by two‑sided Student’s t‑test (two groups) or one‑way ANOVA with Tukey’s post hoc test (multiple groups), unless otherwise specified. ns, not significant; * FDR < 0.05; ** FDR < 0.01; *** FDR < 0.001.

Article Snippet: 786-O cells and OSRC-2 cells were maintained in RPMI Medium 1640 basic (Gibco; C11875500BT) supplemented with fetal bovine serum (FBS, 10 %, PAN; ST30–3302), penicillin–streptomycin–gentamicin (1 %, Solarbio; P1410), and cultured in 95 % air and 5 % CO2 at 37 °C.

Techniques: Knockdown, Migration, In Vitro, Biomarker Discovery, Control